||Recombinant human NUDT16L1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||NUDT16L1, also known Syndesmos, is a cytoplasmic protein that interacts specifically with the cytoplasmic domain of syndecan-4, and it co-localizes with syndecan-4 in focal contacts. This protein interacts with the focal adhesion adaptor protein paxillin. The binding of syndesmos to paxillin is direct, and these interactions are triggered by the activation of protein kinase C.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
||25.5 kDa (231aa) confirmed by MALDI-TOF.
||> 95% by SDS - PAGE.
||0.5 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MSTAAVPELK QISRVEAMRL GPGWSHSCHA MLY AANPGQL FGRIPMRFSV LMQMRFDGLL GFPGGFVDRRFWSLEDGLNR VLG LGL GCLR LTEADYLSSH LTEGPHRVVA HLYARQLTLE QLHAVEISAV HSRDHGLEVL GLVRVPLYTQ KDRVGGFPNFLSNAFVSTAK CQLLFALKVL NMMPEEKLVE ALA AAT EKQK KALEKLLPAS S.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.