||Recombinant human TREX2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||TREX2 is an enzyme containing 3-prime-to-5-prime exonuclease activity and removes mismatched, modified, fragmented, and normal nucleotides to generate the appropriate 3-prime termini for subsequent steps in the DNA metabolic pathways. This enzyme is involved in DNA replication, repair, and recombination.
||Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 30% glycerol.
||28.0 kDa (256aa) confirmed by MALDI-TOF.
||> 95% by SDS - PAGE.
||1 mg/ml (determined by Bradford assay).
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MSEAPRAETF VFLDLEATGL PSVEPEIAEL SLFAV HRSSL ENPEHDESGA LVLPRVLDKL TLCMCPERPF TAKASEITGL SSEGLARCRK AG FDGAVVRT LQAFLSRQAG PICLVAHNGF DYDFPLLCAE LRRLGARLPR DTV CLDTLPA LRGLDRAHSH GTRARGRQGY SLGSLFHRYF RAEPSAAHSA EGD VH TLLLI FLHRAAELLA WADEQARGWA HIEPMYLPPD DPSLEA.
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.