Recombinant Human TPM2 protein, His-tagged

• Cat.No.: TPM2-3442H
• Product Overview: Recombinant Human TPM2(1-284aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Tropomyosin beta chain isoform 2, also known as TPM2, is a member of the actin filament binding protein family which mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 100mM NaCl
• Molecular Mass: 35.1 kDa (304aa) confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSHLEQAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ENRAMKDEEK MELQEMQLKE AKHIAEDSDR KYEEVARKLV ILEGELERSE ERAEVAESRA RQLEEELRTM DQALKSLMAS EEEYSTKEDK YEEEIKLLEE KLKEAETRAE FAERSVAKLE MDAIKKKMQM LKLDKENAID RAEQAEADKK QAEDRCKQLE EEQQALQKKL KGTEDEVEKY SESVKEAQEKKTIDDLEETL ASAKEENVEI HQTLDQTLLE LNNL
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TPM2 tropomyosin 2 (beta) [ Homo sapiens ]
• Official Symbol: TPM2
• Synonyms: TPM2; tropomyosin 2 (beta); AMCD1, arthrogryposis multiplex congenital, distal, type 1; tropomyosin beta chain; DA1; tropomyosin-2; DA2B; NEM4; TMSB; AMCD1
• Gene ID: 7169
• mRNA Refseq: NM_213674
• Protein Refseq: NP_998839
• MIM: 190990
• UniProt ID: P07951
• Chromosome Location: 9p13
• Pathway: Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Dilated cardiomyopathy, organism-specific biosystem; Dilated cardiomyopathy, conserved biosystem; Hypertrophic cardiomyopathy (HCM), organism-specific biosystem; Hypertrophic cardiomyopathy (HCM), conserved biosystem; Muscle contraction, organism-specific biosystem
• Function: actin binding; structural constituent of muscle