Cat.No. : |
MYL9-3445H |
Product Overview : |
Recombinant Human MYL9(1-172aa) fused with His tag at N-terminal was expressed in E. coli. |
Description : |
MYL9, also known as MLC2, is one of the numerous regulatory myosin light chains. Regulatory myosin light chains regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase(MLCK). Phosphorylation of regulatory myosin light chains is catalyzed by MLCK in the presence of calcium and calmodulin and it increases the actin-activated myosin ATPase activity, thereby regulates the contractile activity. |
Source : |
E. coli |
Species : |
Human |
Tag : |
His |
Form : |
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol |
Molecular Mass : |
21.9 kDa (192aa), confirmed by MALDI-TOF |
AA Sequence : |
MGSSHHHHHH SSGLVPRGSHEGMMSEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEASGFIHE DHLRELLTTM GDRFTDEEVD EMYREAPIDK KGNFNYVEFT RILKHGAKDK DD MSSKRAKAKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDEYL |
Purity : |
>90% by SDS - PAGE |
Storage : |
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : |
0.5 mg/ml (determined by Bradford assay) |