||Recombinant human SRI protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||Sorcin, also known as SRI, is a 22-kDa calcium-binding protein initially identified in multidrug-resistant cells. SRI modulates excitation-contraction coupling in the heart and contributes to calcium homeostasis in the heart sarcoplasmic reticulum.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.2M NaCl, 1mM DTT, 0.1mM PMSF
||24.1 kDa (221aa) confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSMAYPGHP GAGGGYYPGG YGGAPGGPAF PGQTQDPLYG YFAAVAGQDG QIDADELQRC LTQSGIAGGY KPFNLETCRL MVSMLDRDMS GTMGFNEFKE LWAVLNGWRQ HFISFDTDRS GTVDPQELQK ALTTMGFRLS PQAVNSIAKR YSTNGKITFD DYIACCVKLR ALTDSFRRRD TAQQGVVNFP YDDFIQCVMS V
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.