||Recombinant human GRPEL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =23.6 kDa (211aa) confirmed by MALDI-TOF.
||GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MCTATKQKNS GQNLEEDMGQ SEQKADPPAT EKTLLEEKVK LEEQLKETVE KYKRALADTE NLRQRSQKLV EEAKLYGIQA FCKDLLEVAD VLEKATQCVP KEEIKDDNPH LKNLYEGLVM TEVQIQKVFT KHGLLKLNPV GAKFDPYEHE ALFHTPVEGK EPGTVALVSK VGYKLHGRTL RPALVGVVKE A
||> 90% by SDS – PAGE.
||Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.