||Recombinant humanMAPRE3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 34.1 kDa (301aa) confirmed by MALDI-TOF.
||MAPRE1 is a microtubule associated protein that interacts with the colorectal adenomatous polyposis coli tumor suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability. This protein is related to MAPRE1 and likewise associates with the microtubule cytoskeleton. MAPRE3 is expressed predominantly in the central nervous system and preferentially associates with APCL, a homolog of the adenomatous polyposis coli tumor suppressor protein.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
||> 90% by SDS – PAGE.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 2mM DTT, 0.1M NaCl.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.