"MCEE" Related Products

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Recombinant Human Methylmalonyl CoA Epimerase, His-tagged

Cat. No.: MCEE-801H
Product Overview: Recombinant human MCEE protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 17.3 kDa (161aa) confirmed by MALDI-TOF.
Description: MCEE catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids, odd chain-length fatty acids, and other metabolites. This protein deficiency is an autosomal recessive inborn error of amino acid metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria may present in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma.
Source: E.coli.
Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MQVTGSVWNL GRLNHVAIAV PDLEKAAAFY KNILGAQVSE AVPLPEHGVS VVFVNLGNTK MELLHPLGRD SPIAGFLQKN KAGGMHHICI EVDNINAAVM DLKKKKIRSL SEEVKIGAHG KPVIFLHPKD CGGVLVELEQ A
Purity: > 90% by SDS – PAGE.
Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 0.1mM PMSF, 10% glycerol.
Concentration: 1 mg/ml (determined by Bradford assay).
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: MCEE methylmalonyl CoA epimerase [ Homo sapiens ]
Synonyms: MCEE; methylmalonyl CoA epimerase; GLOD2; methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; glyoxalase domain containing 2; EC 5.1.99.1
Gene ID: 84693
mRNA Refseq: NM_032601
Protein Refseq: NP_115990
MIM: 608419
UniProt ID: Q96PE7
Chromosome Location: 2p13.3
Pathway: Metabolic pathways; Propanoate metabolism; Valine, leucine and isoleucine degradation
Function: isomerase activity; methylmalonyl-CoA epimerase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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