||Recombinant human PSME1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 30.8kDa (269aa) confirmed by MALDI-TOF.
||PSME1 is an interferon gamma (IFNG) inducible proteasome activator required for presentation of certain major histocompatibility (MHC) class I antigens. The PSME1 complex is an alternative proteasome activator that does not employ the use of ubiquitin. The PSME1 complex is composed of two homologous subunits, alpha and beta, which have similar catalytic properties and associate to form a hexameric ring.
|Sequences Of Amino Acids:
||MGSSHHHHHH SSGLVPRGSH MAMLRVQPEA QAKVDVFRED LCTKTENLLG SYFPKKISEL DAFLKEPALN EANLSNLKAP LDIPVPDPVK EKEKEERKKQ QEKEDKDEKK KGEDEDKGPP CGPVNCNEKI VVLLQRLKPE IKDVIEQLNL VTTWLQLQIP RIEDGNNFGV AVQEKVFELM TSLHTKLEGF HTQISKYFSE RGDAVTKAAK QPHVGDYRQL VHELDEAEYR DIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY
||> 90% by SDS – PAGE.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl.
||0.5 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.