"KIT" Related Products

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Recombinant Human KIT, His-tagged

Cat. No.: KIT-540H
Product Overview: Recombinant human c-KIT was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW = 50 kDa.
Description: c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. c-KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in c-KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed curbing neoplastic mast cell growth.
Source: Sf9 insect cells.
Purity: >80% by SDS-PAGE.
Specific Activity: 15 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant cKit.
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Stability: >6 months at –80°C.
Gene Name: KIT v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog [ Homo sapiens ]
Synonyms: KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; soluble KIT variant 1; mast/stem cell growth factor receptor; proto-oncogene tyrosine-protein kinase Kit; EC 2.7.10.1
Gene ID: 3815
mRNA Refseq: NM_000222
Protein Refseq: NP_000213
MIM: 164920
UniProt ID: P10721
Chromosome Location: 4q11-q12
Pathway: Acute myeloid leukemia; Cytokine-cytokine receptor interaction; Endocytosis; Hematopoietic cell lineage; Melanogenesis; Pathways in cancer
Function: ATP binding; nucleotide binding; protein binding; receptor signaling protein tyrosine kinase activity; stem cell factor receptor activity; transferase activity

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