||Recombinant Human PLK1 (Accession # NM_005030) , fused with an N-terminal His tag, was produced in baculovirus in Sf 9 insect cells.
||Sf 9 insect cells
||Supplied in 50 mM sodium phosphate (pH 7.0), 300 mM NaCl, 0.25 mM DTT, 150 mM imidazole, 0.1 mM PMSF, and 25% glycerol.
||The specific activity of PLK1 was determined to be 20 nmol/min/mg using a dephospho-casein substrate
||In SDS-PAGE migrates as 70 kDa.
||The purity was determined to be >95% by densitometry
||Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).