||Recombinant human PTP4A2 protein(2-167) with N-terminal His tag was expressed in anE.coliexpression system, MW= 19.1 kDa.
||Protein tyrosine phosphatase type IVA 2 is an enzyme that in humans is encoded by the PTP4A2 gene. he protein encoded by this gene belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity.
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol, and 3 mM DTT.
||0.334 pmol/min/μg, Assay conditions: enzyme was incubated in50mM Tris pH7.4, 150mM NaCl 5mM DTT, and 12.5mM PNPP as a substrate for 2h at 37°C. Absorbance was read by Tecan spectraphotometer at 405 nm wavelength.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
||>6 months at –80°C.