Recombinant Human Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G, His-tagged

• Cat.No.: PPM1G-1756H
• Product Overview: Recombinant humanPPM1G protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: PPM1G-1756H
• Description: PPM1G, as known asprotein phosphatase 1G, is a member of the PP2C family of Ser/Thr proteinphosphatases that are negative regulators of cell stress response pathways.This enzyme localizes to the cytoplasm and is widely expressed, with mostabundant expression detected in testis, skeletal muscle and heart. It isnecessary for the dephosphorylation of pre-mRNA splicing factors, which is animportant process for the formation of the functional spliceosome.
• Concentration: 1 mg/ml
• Source: Human
• Host: E.coli
• Form: Supplied as a liquidin 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl, 1mM DTTand 0.1mM PMSF.
• Purity: > 85% by SDS -PAGE
• Sequence: 1-546 amino acids:MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELDSETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGRPTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEAGPEDS
• Molecular Mass: 61.4 kDa (566aa)
• Applications: SDS-PAGE
• Storage: Store at 4 deg C forshort term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg Cfor long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: PPM1G

Gene Information

• Gene Name: PPM1Gprotein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform [ Homosapiens ]
• Synonyms: PPM1G; proteinphosphatase, Mg2+/Mn2+ dependent, 1G; PPM1C; PP2CG; PPP2CG; MGC1675; MGC2870;PP2CGAMMA; PP2Cgamma; PP2C-gamma; PP2C, gamma; EC 3.1.3.16;OTTHUMP00000123426; protein phosphatase 1G; Protein phosphatase 1G; proteinphosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2Cisoform gamma; Protein phosphatase 2C isoform gamma; Protein phosphatasemagnesium-dependent 1 gamma; protein phosphatase magnesium-dependent 1 gamma;protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase1G (formerly 2C), magnesium-dependent, gamma isoform
• Gene ID: 5496
• mRNA Refseq: NM_002707
• Protein Refseq: NP_002698
• MIM: 605119
• UniProt ID: O15355
• Chromosome Location: 2p23.3
• Pathway: mRNA processing
• Function: proteinserine/threonine phosphatase activity; manganese ion binding; magnesium ionbinding; hydrolase activity