"PPM1G" Related Products

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Recombinant Human Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G, His-tagged

Cat. No.: PPM1G-1756H
Product Overview: Recombinant humanPPM1G protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
Description: PPM1G, as known asprotein phosphatase 1G, is a member of the PP2C family of Ser/Thr proteinphosphatases that are negative regulators of cell stress response pathways.This enzyme localizes to the cytoplasm and is widely expressed, with mostabundant expression detected in testis, skeletal muscle and heart. It isnecessary for the dephosphorylation of pre-mRNA splicing factors, which is animportant process for the formation of the functional spliceosome.
Concentration: 1 mg/ml
Source: Human
Host: E.coli
Form: Supplied as a liquidin 20mM Tris-HCl buffer, pH 8.0, containing 20% glycerol, 0.1M NaCl, 1mM DTTand 0.1mM PMSF.
Purity: > 85% by SDS -PAGE
Sequence: 1-546 amino acids:MGSSHHHHHH SSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELDSETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGRPTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEAGPEDS
Molecular Mass: 61.4 kDa (566aa)
Applications: SDS-PAGE
Storage: Store at 4 deg C forshort term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg Cfor long term storage. Avoid repeated freeze/thaw cycles.
OfficialSymbol: PPM1G
Gene Name: PPM1Gprotein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform [ Homosapiens ]
Synonyms: PPM1G; proteinphosphatase, Mg2+/Mn2+ dependent, 1G; PPM1C; PP2CG; PPP2CG; MGC1675; MGC2870;PP2CGAMMA; PP2Cgamma; PP2C-gamma; PP2C, gamma; EC 3.1.3.16;OTTHUMP00000123426; protein phosphatase 1G; Protein phosphatase 1G; proteinphosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2Cisoform gamma; Protein phosphatase 2C isoform gamma; Protein phosphatasemagnesium-dependent 1 gamma; protein phosphatase magnesium-dependent 1 gamma;protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase1G (formerly 2C), magnesium-dependent, gamma isoform
Gene ID: 5496
mRNA Refseq: NM_002707
Protein Refseq: NP_002698
MIM: 605119
UniProt ID: O15355
Chromosome Location: 2p23.3
Pathway: mRNA processing
Function: proteinserine/threonine phosphatase activity; manganese ion binding; magnesium ionbinding; hydrolase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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