Recombinant Human TEK Tyrosine Kinase, Endothelial, His-tagged
Cat.No. : | TEK-547H |
Product Overview : | Recombinant human TIE 2 was expressed bybaculovirus in Sf9 insect cellsusing an N-terminal GST tag. MW = 61 kDa. |
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- Gene Information
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Cat. No. : | TEK-547H |
Description : | TIE 2 or TEK is a receptor tyrosine kinase that is expressed principally on vascular endothelium. Disrupting TIE 2 function in mice results in embryonic lethality with defects in embryonic vasculature, suggesting a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TIE 2 receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TIE 2 kinase domain and are part of the cellular responses that mediate TIE 2 function. TIE 2 expression is upregulated in the endothelium of vascular "hot spots" in human breast cancer specimens. However, TIE 2 is also overexpressed in areas of active angiogenesis in normal tissues. |
Source : | Baculovirus infected Sf9 cells. |
Purity : | >60% by SDS-PAGE. |
Specific Activity : | 140 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substarte per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug/ml recombinant Tie2. |
Application : | Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
Formulated In : | 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT. |
Stability : | >6 months at –80°C. |
Gene Name : | TEK TEK tyrosine kinase, endothelial [ Homo sapiens ] |
Synonyms : | TEK; TEK tyrosine kinase, endothelial; TIE2; VMCM; TIE-2; VMCM1; CD202B; soluble TIE2 variant 1; soluble TIE2 variant 2; p140 TEK |
Gene ID : | 7010 |
mRNA Refseq : | NM_000459 |
Protein Refseq : | NP_000450 |
MIM : | 600221 |
UniProt ID : | Q02763 |
Chromosome Location : | 9p21 |
Pathway : | Hemostasis; Signaling in Immune system |
Function : | ATP binding; nucleotide binding; protein binding; receptor activity; transferase activity; transmembrane receptor protein tyrosine kinase activity |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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