||Recombinant humanFGF10 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||FGF10, also known asfibroblast growth factor 10, exhibits mitogenic activity for keratinizingepidermal cells, but essentially no activity for fibroblasts, which issimilar to the biological activity of FGF7. Studies of the mouse homolog ofsuggested that this gene is required for embryonic epidermal morphogenesisincluding brain development, lung morphogenesis, and initiation of lim budformation. FGF10 is also implicated to be a primary factor in the process ofwound healing.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 50% glycerol, 0.2M NaCl, 2mM DTT, 2mM EDTA.
||22.0 kDa(196aa), confirmed by MALDI-TOF.
||> 95%by SDS - PAGE
||0.25mg/ml(determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHHSSGLVPRGSH MGSHMQALGQ DMVSPEATNS SSSSFSSPSS AGRHVRSYNHLQGDVRWRKL FSFTKYFLKI EKNGKVSGTK KENCPYSILE ITSVEIGVVA VKAINSNYYL AMNKKGKLYGSKEFNNDCKL KERIEENGYN TYASFNWQHN GRQMYVALNG KGAPRRGQKT RRKNTSAHFL PMVVHS
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.