Recombinant Human Vascular Endothelial Growth Factor A

• Cat.No.: VEGFA-5532H
• Product Overview: Recombinant human VEGF189,a 21 kDa protein consisting of 189 amino acid residues, is produced as ahomodimer.

Specification

• Cat. No.: VEGFA-5532H
• Description: This gene is amember of the platelet-derived growth factor (PDGF)/vascular endothelialgrowth factor (VEGF) family and encodes a protein that is often found as adisulfide linked homodimer. This protein is a glycosylated mitogen thatspecifically acts on endothelial cells and has various effects.
• Source: E. coli
• Species: Human
• Form: Lyophilized
• Purity: > 98% asdetermined by SDS-PAGE analysis and visualised by silver stain.
• Molecular Mass: 42 kDa
• Endotoxin Level: < 0.1 ng/μg ofVEGF189
• Biological Activity: Determined by thestimulation of 3H-thymidine incorporation and cell proliferation by humanumbilical vein endothelial cells for VEGF189. The ED50 is in therange of 1-2 ng/ml.
• Reconstitution: Centrifuge vialprior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration not less than 0.1-1.0 mg/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions.
• Storage: Store lyophilizedprotein at -20°C to -80°C and reconstituted protein in working aliquots at-20°C. Avoid repeated freeze-thaw cycles.
• OfficialSymbol: VEGFA
• Pathways: Bladder cancer;Cytokine-cytokine receptor interaction; Endochondral Ossification; Focaladhesion; Glypican 1 network; HIF-1-alpha transcription factor network;HIF-2-alpha transcription factor network; Heart Development; Hemostasis;Hypertrophy Model; Id Signaling Pathway; Integrins in angiogenesis;Neurophilin interactions with VEGF and VEGFR; Pancreatic cancer; Pathways incancer

Gene Information

• Gene Name: VEGFA vascular endothelialgrowth factor A [ Homo sapiens ]
• Synonyms: VEGFA;vascular endothelial growth factor A; VPF; VEGF; MVCD1; vascularendothelial growth factor A; OTTHUMP00000016487; OTTHUMP00000016488;OTTHUMP00000165985; OTTHUMP00000165986; OTTHUMP00000165987;OTTHUMP00000224107; OTTHUMP00000224108; OTTHUMP00000224109; OTTHUMP00000224153;OTTHUMP00000224154; OTTHUMP00000224423; OTTHUMP00000224424;OTTHUMP00000224425; OTTHUMP00000224426; OTTHUMP00000224427;OTTHUMP00000224428; OTTHUMP00000224429; OTTHUMP00000224430; vascularpermeability factor; Vascular endothelial growth factor A; Vascular permeability factor
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• MIM: 192240
• UniProt ID: P15692
• Chromosome Location: 6p12
• Function: cell surfacebinding; chemoattractant activity; cytokine activity; extracellular matrixbinding; fibronectin binding; growth factor activity; heparin binding;platelet-derived growth factor receptor binding; protein binding; proteinheterodimerization activity; vascular endothelial growth factor receptor 1binding; vascular endothelial growth factor receptor 2 binding; vascularendothelial growth factor receptor binding