||Recombinant human HDHD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 27.4 kDa (248aa).
||HDHD1, also known as haloacid dehalogenase-like hydrolase domain-containing potein 1, belong to the adiponutrin family. This protein is of interest because it is an X-linked gene that escapes X-inactivation. It is particularly important in the Understanding of human X chromosome structural organization as well as the Mechanism of X-inactivation.
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAAPPQPVTH LIFDMDGLLL DTERLYSVVF QEICNRYDKK YSWDVKSLVM GKKALEAAQI IIDVLQLPMS KEELVEESQT KLKEVFPTAA LMPGAEKLII HLRKHGIPFA LATSSGSASF DMKTSRHKEF FSLFSHIVLG DDPEVQHGKP DPDIFLACAK RFSPPPAMEK CLVFEDAPNG VEAALAAGMQ VVMVPDGNLS RDLTTKATLV LNSLQDFQPE LFGLPSYE
||Liquid. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 20% glycerol
||> 95% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.