Active Recombinant Mouse IL18BP, His tagged

• Cat.No.: IL18BP-767M
• Product Overview: Recombinant Mouse IL18BP precursor (NP_034661.1) (Met 1-Ala 193), fused with the polyhistide tag at the C-terminus, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized IL18BP Protein, Mouse, (His Tag) at 2 μg/mL (100 μL/well) can bind IL-18 Protein, Mouse Biotinylated, the EC50 of Biotinylated Mouse IL-18 is 25-80 μg/mL.
• Molecular Mass: The recombinant mouse IL18BP comprises 176 amino acids and has a predicted molecular mass of 19.4 kDa. In SDS-PAGE under reducing conditions, rm IL18BP migrates between 35-50 kDa due to different glycosylation.
• Protein Length: Met 1-Ala 193
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publication:
  Production of IL-18 Binding Protein by Radiosensitive and Radioresistant Cells in CpG-Induced Macrophage Activation Syndrome (2020)
  Detection of Free Bioactive IL-18 and IL-18BP in Inflammatory Disorders (2023)
  Interleukin-18 as a Therapeutic Target in Western-diet Induced Cardiomyopathy (2017)

Gene Information

• Gene Name: Il18bp interleukin 18 binding protein [ Mus musculus ]
• Official Symbol: IL18BP
• Synonyms: IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; interferon gamma inducing factor binding protein; interferon gamma-inducing factor-binding protein; MC54L; Igifbp; IL-18BP
• Gene ID: 16068
• mRNA Refseq: NM_010531
• Protein Refseq: NP_034661