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Recombinant Mouse Glia Maturation Factor, Beta

Cat. No.: Gmfb-1885M
Product Overview: RecombinantMouse Gmfb produced in E. coli is a singlenon-glycosylated polypeptide chain containing 141 amino acids.
Description: Gmfb, abrain-specific protein that belongs to the actin-binding proteins (ADF)structural family. Gmfb appears to play a role in the differentiation,maintenance, and regeneration of the nervous system. It also supports theprogression of certain autoimmune diseases, possibly through its ability toinduce the production and secretion of various proinflammatory cytokines.Mouse Gmfb shows more than 90% identity with human Gmfb.
Source: E. coli
EndotoxinLevel: Less than1EU/μg of rMuGmfb as determined by LAL method.
Molecular Weight: Approximately16.6 kDa
Form: Lyophilizedfrom a 0.2 μmfiltered concentrated solution in PBS, pH 7.4.
Purity: > 97%by SDS-PAGE gel and HPLC analyses.
Reconstitution: We recommend that this vial bebriefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSAto a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportionedinto working aliquots and stored at ≤-20°C.Further dilutions should be made in appropriate buffered solutions.
Amino Acid Sequence: SESLVVCDVA EDLVEKLRKF RFRKETHNAAIIMKIDKDER LVVLDEELEG VSPDELKDEL PERQPRFIVY SYKYQHDDGR VSYPLCFIFS SPVGCKPEQQ MMYAGSKNKLVQTAELTKVF EIRNTEDLTE EWLREKLGFF H
Storage: Thislyophilized preparation is stable at 2-8°C, butshould be kept at -20°C for long term storage, preferably desiccated. Uponreconstitution, the preparation is stable for up to one week at 2-8°C. Formaximal stability, apportion the reconstituted preparation into workingaliquots and store at -20°C to -70°C. Avoid repeatedfreeze/thaw cycles.
OfficialSymbol: Gmfb
nullValue_other:
Chromosome Location14
Gene Name: Gmfbglia maturation factor, beta [ Mus musculus ]
Synonyms: Gmfb; glia maturation factor, beta; C79176; AI851627;D14Ertd630e; 3110001H22Rik; 3110001O16Rik; GMF-beta; OTTMUSP00000028260
Gene ID: 63985
mRNA Refseq: NM_022023
Protein Refseq: NP_071306
UniProt ID: Q9CQI3

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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