||Recombinant PPIF protein, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques, 22040Da (207aa).
||Peptdyl-prolyl cis-trans isomerase, mitochondrial is anenzymethat in humans is encodeid by the PPIFgene. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.
|Sequences of amino acids:
||MLALRCGSRW LGLLSVPRSV PLRLPAARAC SKGSGDPSSS SSSGNPLVYL DVDANGKPLG RVVLELKADV VPKTAENFRA LCTGEKGFGY KGSTFHRVIP SFMCQAGDFT NHNGTGGKST YGSRFPDENT TLKHVGPGVL SMANAGPNTN GSQFFICTIK TDWLDGKHVV FGHVKEGMDY VKKIESFGSK SGRTSKKIVI TDCGQLS
||10 mM Tris. pH 8.0. 0.1% Triton X-100. 0.002% NaN3. 10mM DTT
||95% by SDS - PAGE