| Description : |
Neuron-specific Enolase (Enolase 2) is a 47.1 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Neuron-specific Enolase is 433 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Neuron-specific Enolase exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both murine and canine Enolase 2. It shares 83 % aa identity with human enolases 1 and 3. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose. |
| Molecular Mass : |
Approximately 47.1 kDa, a single non-glycosylated polypeptide chain containing 433 amino acids. |
| Protein length : |
433 |
| AA Sequence : |
SIEKIWAREILDSRGNPTVEVDLYT AKGLFRAAVPSGASTGIYEALELRD GDKQRYLGKGVLKAVDHINSTIAPA LISSGLSVVEQEKLDNLMLELDGTE NKSKFGANAILGVSLAVCKAGAAER ELPLYRHIAQLAGNSDLILPVPAFN VINGGSHAGNKLAMQEFMILPVGAE SFRDAMRLGAEVYHTLKGVIKDKYG KDATNVGDEGGFAPNILENSEALEL VKEAIDKAGYTEKIVIGMDVAASEF YRDGKYDLDFKSPTDPSRYITGDQL GALYQDFVRDYPVVSIEDPFDQDDW AAWSKFTANVGIQIVGDDLTVTNPK RIERAVEEKACNCLLLKVNQIGSVT EAIQACKLAQENGWGVMVSHRSGET EDTFIADLVVGLCTGQIKTGAPCRS ERLAKYNQLMRIEEELGDEARFAGH NFRNPSVL |
| Endotoxin : |
Less than 0.1 EU/µg of rHuNSE as determined by LAL method. |
| Purity : |
>96% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
