Recombinant Human MHC Class I Polypeptide-Related Sequence B

• Cat.No.: MICB-5454H
• Product Overview: Recombinant Humanproduced in E. coli is a single, non-glycosylated polypeptide chaincontaining 326 amino acids and having a MW 37 kDa. The sequence contains theextracellular domain of the mature human MICB (amino acid residues Ala23-Tyr312).

Specification

• Cat. No.: MICB-5454H
• Description: MICB is aprotein that in humans is encoded by the MICB gene. This gene encodes aheavily glycosylated protein which is a ligand for the NKG2D type IIreceptor. Binding of the ligand activates the cytolytic response of naturalkiller (NK) cells, CD8 alphabeta T cells, and gammadelta T cells whichexpress the receptor. This protein is stress-induced and is similar to MHCclass I molecules; however, it does not associate with beta-2-microglobulinor bind peptides.
• Source: E. coli
• Species: Human
• Form: Lyophilized from aconcentrated (1 mg/ml) solution containing no additives.
• Purity: Greaterthan 95.0% as determined by analysis by RP-HPLC, anion-exchange FPLC and byreducing and non-reducing SDS-PAGE Silver Stained gel.
• Endotoxin Level: Less than0.1 ng/μg (1 EU/μg) of Recombinant Human MICB.
• Biological Activity: ProSpec'sRecombinant Human MICB is fully biologically active when compared to standards.Measured by its ability to bind MICB antibody in ELISA.
• Reconstitution: It isrecommended to reconstitute the lyophilized MICB in sterile 18 MΩ-cm H2O notless than 100 μg/ml, which can then be further diluted to other aqueoussolutions.
• storage: LyophilizedRecombinant Human MICB although stable at room temperature for 3 weeks,should be stored desiccated below -20°C. Upon reconstitution MICB should be storedat 2-4°C between 2-7 days and for future use below -20°C. For long termstorage it is recommended to add a carrier protein (0.1% HSA or BSA). Pleaseavoid freeze-thaw cycles.
• OfficialSymbol: MICB
• Pathways: Antigen processing andpresentation; Cell adhesion molecules (CAMs); Natural killer cell mediatedcytotoxicity; Type I diabetes mellitus; Signaling in Immune system

Gene Information

• Gene Name: MICB MHC class Ipolypeptide-related sequence B [ Homo sapiens ]
• Synonyms: MICB; MHC class Ipolypeptide-related sequence B; PERB11.2; MICB; MIC-B; OTTHUMP00000029089;MHC class I polypeptide-related sequence B; MHC class I molecule; MHC class Imic-B antigen; stress inducible class I homolog; MHC class I chain-relatedprotein B; MHC class I-like molecule PERB11.2-IMX; OTTHUMP00000035091;TTHUMP00000035588
• Gene ID: 4277
• mRNA Refseq: NM_005931
• Protein Refseq: NP_005922
• MIM: 602436
• UniProt ID: Q0EFB4
• Chromosome Location: 6p21.3
• Function: natural killer cell lectin-like receptorbinding