||Recombinant human PBK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventionalchromatography techniques.
||PBK is aserine/threonine kinase related to the dual specific mitogen-activatedprotein kinase (MAPKK) family. PBK is abundant in placenta and absent fromadult brain tissue. A PDZ domain in the tumor suppressor protein Dlg cancoordinate with the T/SXV motif of PBK. This protein may be involved in theactivation of lymphoid cells and support testicular functions, with asuggested role in the process of spermatogenesis.
||Liquid.In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
||38.6 kDa(346aa) confirmed by MALDI-TOF
||> 95%by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNVYLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPNIVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHGDIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLTLWEMMTLSIPHINLSNDDDDEDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoidrepeated freezing and thawing cycles.