||Recombinant humanPGAM2 protein,fused to His-tag at N-terminus, was expressed in E. coli and purified byusing conventional chromatography.
||PGAM2, also known asphosphoglyceratemutase 2, belongs to the phosphoglycerate mutase family.Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolyticpathway. The PGAM is a dimeric enzyme containing, in different tissues,different proportions of a slow-migrating muscle (MM) isozyme, afast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodesmuscle-specific PGAM subunit. Mutations in this gene cause musclephosphoglycerate mutase eficiency, also known as glycogen storage disease X.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
||30.9 kDa (273aa)confirmed by MALDI-TOF
||> 95%by SDS-PAGE
||1 mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKMEFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFDIPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSLRGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG KAK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoidrepeated freezing and thawing cycles.
||Gluconeogenesis; Glucose metabolism;Glycolysis; Glycolysis (Embden-Meyerhof pathway); Glycolysis / Gluconeogenesis; Glycolysisand Gluconeogenesis; Metabolic pathways; MetRabolism of carbohydrates