||Recombinant humanPSMB7 protein,fused to His-tag at N-terminus, was expressed in E. coli and purified by usingconventional chromatography.
||PSMB7, also known asproteasome subunit beta type-7, is a multicatalytic proteinasecomplex with a highly ordered ring-shaped 20S core structure. The corestructure is composed of 4 rings of 28 non-identical subunits; 2 rings arecomposed of 7 alpha subunits and 2 rings are composed of 7 beta subunits.Proteasomes are distributed throughout eukaryotic cells at a highconcentration and cleave peptides in an ATP/ubiquitin-dependent process in anon-lysosomal pathway. An essential function of a modified proteasome, theimmunoproteasome, is the processing of class I MHC peptides. This geneencodes a member of the proteasome B-type family, also known as the T1Bfamily that is a 20S core beta subunit in the proteasome.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT.
||27.6 kDa (255aa)confirmed by MALDI-TOF
||> 80%by SDS-PAGE
||0.25 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MTTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTAADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGSTDKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPYTVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoidrepeated freezing and thawing cycles.
||APC/C-mediated degradation of cell cycleproteins; APC/C:Cdc20 mediated degradation of Securin; APC/C:Cdc20 mediateddegradation of mitotic proteins; Adaptive Immune System; Antigenprocessing-Cross presentation; Apoptosis; Assembly of the pre-replicativecomplex; Cell Cycle Checkpoints; Cell Cycle; DNA Replication; DNA ReplicationPre-Initiation; ER-Phagosome pathway; G1/S DNA Damage Checkpoints; G1/STransition