"PPP3R2" Related Products

Recombinant Human Protein Phosphatase 3, Regulatory Subunit B, His-tagged

Cat. No.: PPP3R2-973H
Product Overview: Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description: PPP3R2 is a calcium-dependent, calmodulin stimulated serine/threonine protein phosphatase. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Recombinant human PPP3R2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Formulation: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT.
Molecular Weight: 22 kDa (193aa), confirmed by MALDI-TOF
Purity: > 95% by SDS – PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay)
Sequences of animo acids: MGSSHHHHHH SSGLVPRGSHMSTMGNEASY PAEMCSHFDN DEIKRLGRRF KKLDLDKSGS LSVEEFMSLP ELRHNPLVRR VIDVFDTDGD GEVDFKEFIL GTSQFSVKGD EEQKLRFAFSIYDMDKDGYI SNGELFQVLK MMVGNNLTDW QLQQLVDKTI IILDKDGDGK ISFEEFSAVV RDLEIHKKLV LIV
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
Gene Name: PPP3R2 protein phosphatase 3, regulatory subunit B, beta [ Homo sapiens ]
Synonyms: PPP3R2;PPP3RL; protein phosphatase 3, regulatory subunit B, beta; Calcineurin subunit B type 2;Calcineurin B-like protein; Calcineurin BII; Protein phosphatase 2B regulatory subunit 2; Protein phosphatase 3 regulatory subunit B beta isoform; CBLP; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), beta isoform (calcineurin B, type II);protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform
Gene ID: 5535
mRNA Refseq: NM_147180
Protein Refseq: NP_671709
UniProt ID: Q96LZ3
Chromosome Location: 9q31
Function: calcium ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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