"TPK1" Related Products

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Recombinant Human Thiamin Pyrophosphokinase 1, His-tagged

Cat. No.: TPK1-1937H
Product Overview: Recombinant humanTPK1 protein,fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography.
Description: TPK1, also known as thiamin pyrophosphokinase 1, catalyzes thephosphorylation of thiamine to thiamine pyrophosphate. This protein can alsocatalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20%glycerol, 1mM DTT.
Molecular Weight: 29.8 kDa (267aa)confirmed by MALDI-TOF.
Purity: > 90%by SDS - PAGE
Concentration: 1 mg/ml (determinedby Bradford assay)
Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACADGGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKDLKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLIPVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS
Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
Pathways: Metabolic pathways; Metabolism;Metabolism of vitamins and cofactors; Metabolism of water-soluble vitaminsand cofactors; Thiamine metabolism; Vitamin B1 (thiamin) metabolism
Gene Name: TPK1thiamin pyrophosphokinase 1 [ Homo sapiens ]
Official Symbol: TPK1
Synonyms: TPK1;thiamin pyrophosphokinase 1; PP20; HTPK1; thiaminpyrophosphokinase 1; thiamine kinase; placental protein 20; thiamine diphosphokinase; Putative uncharacterized protein DKFZp781C0719; DKFZp781C0719
Gene ID: 27010
mRNA Refseq: NM_001042482
Protein Refseq: NP_001035947
MIM: 606370
UniProt ID: Q68DW6
Chromosome Location: 7q34-q35
Function: ATP binding; kinaseactivity; nucleotide binding; thiamine diphosphokinase activity; transferaseactivity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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