||Recombinant humanTPK1 protein,fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography.
||TPK1, also known as thiamin pyrophosphokinase 1, catalyzes thephosphorylation of thiamine to thiamine pyrophosphate. This protein can alsocatalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20%glycerol, 1mM DTT.
||29.8 kDa (267aa)confirmed by MALDI-TOF.
||> 90%by SDS - PAGE
||1 mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACADGGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKDLKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLIPVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
||Metabolic pathways; Metabolism;Metabolism of vitamins and cofactors; Metabolism of water-soluble vitaminsand cofactors; Thiamine metabolism; Vitamin B1 (thiamin) metabolism