"GATM" Related Products

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Recombinant Human Low Density Lipoprotein Receptor-Related Protein Associated Protein 1, His-tagged

Cat. No.: GATM-1925H
Product Overview: Recombinant humanGATM protein, fused to His-tag at N-terminus, was expressed in E. coli and purifiedby using conventional chromatography techniques.
Description: GATM(Glycine amidinotransferase, mitochondrial) is a mitochondrial enzyme thatbelongs to the amidinotransferase family. This enzyme is involved in creatinebiosynthesis, whereby it catalyzes the transfer of a guanido group fromL-arginine to glycine, resulting in guanidinoacetic acid, the immediateprecursor of creatine. Creatine plays a vital role in energy metabolism inmuscle tissues. It plays a role in embryonic and central nervous systemdevelopment.
Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 200mM NaCl.
Molecular Weight: 46.9kDa(410aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appearhigher).
Purity: > 90%by SDS-PAGE
Concentration: 1.0mg/ml (determined by Bradford assay)
Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSMSTQAAT ASSRNSCAAD DKATEPLPKD CPVSSYNEWD PLEEVIVGRAENACVPPFTI EVKANTYEKY WPFYQKQGGH YFPKDHLKKA VAEIEEMCNI LKTEGVTVRR PDPIDWSLKYKTPDFESTGL YSAMPRDILI VVGNEIIEAP MAWRSRFFEY RAYRSIIKDY FHRGAKWTTA PKPTMADELYNQDYPIHSVE DRHKLAAQGK FVTTEFEPCF DAADFIRAGRDIFAQRSQVT NYLGIEWMRR HLAPDYRVHI ISFKDPNPMH IDATFNIIGP GIVLSNPDRP CHQIDLFKKAGWTIITPPTP IIPDDHPLWM SSKWLSMNVL MLDEKRVMVD ANEVPIQKMF EKLGITTIKV NIRNANSLGGGFHCWTCDVR RRGTLQSYLD
Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways: Arginine and proline metabolism; Creatinemetabolism; Creatine pathway; Glycine; Metabolic pathways; Metabolism;Metabolism of amino acids and derivatives; Urea cycle and metabolism of aminogroups
Gene Name: GATM glycine amidinotransferase(L-arginine:glycine amidinotransferase) [ Homo sapiens ]
Official Symbol: GATM
Synonyms: GATM; glycine amidinotransferase(L-arginine:glycine amidinotransferase); AT; AGAT; glycineamidinotransferase, mitochondrial; transamidinase; L-arginine:glycineamidinotransferase; EC 2.1.4.1
Gene ID: 2628
mRNA Refseq: NM_001482
Protein Refseq: NP_001473
MIM: 602360
UniProt ID: P50440
Chromosome Location: 15q21.1
Function: glycine amidinotransferase activity;transferase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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