||Recombinant humanGPX3(U73C) protein, fused to His-tag at N-terminus, was expressed in E. coliand purified by using conventional chromatography.
||GPX3,also known as glutathione peroxidase 3, belongs to the glutathione peroxidasefamily, which functions in the detoxification of hydrogen peroxide. GPX3protects cells and enzymes from oxidative damage, by catalyzing the reductionof hydrogen peroxide, lipid peroxides and organic hydroperoxide, byglutathione. This protein is one of only a few proteins known in highervertebrates to contain selenocysteine, which occurs at the active site ofglutathione peroxidase and is coded by the nonsense (stop) codon TGA.
||Liquid. 20mMTris-HCl buffer (pH7.5) containing 40% glycerol, 0.15M NaCl, 1mM DTT.
||25.7 kDa(227aa) confirmed by MALDI-TOF.
||> 85%by SDS-PAGE
||0.25 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MQSRGQEKSK MDCHGGISGT IYEYGALTID GEEYIPFKQY AGKYVLFVNVASYCGLTGQY IELNALQEEL APFGLVILGF PCNQFGKQEP GENSEILPTL KYVRPGGGFV PNFQLFEKGDVNGEKEQKFY TFLKNSCPPT SELLGTSDRL FWEPMKVHDI RWNFEKFLVG PDGIPIMRWH HRTTVSNVKMDILSYMRRQA ALGVKRK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Arachidonic acid metabolism; FolateMetabolism; Glutathione metabolism; Oxidative Stress; Selenium Metabolism andSelenoproteins; Selenium Pathway; glutathione redox reactions I