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Recombinant Human Phenylalanyl-tRNA Synthetase 2, His-tagged

Cat. No.: FARS2-976H
Product Overview: Recombinant human FARS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description: Phenylalanyl-tRNAsynthetase 2, mitochondrial (PheRS), also known as FARS2, belongs to the class II aminoacyl- tRNAsynthetase family. FARS2 is a mitochondrial matrix protein. Functioning as a monomer, FARS2 catalyzes the ATP-dependent conversion of L-phenylalanine and tRNA (Phe) to L-phenlalanyltRNA (Phe), an event that is crucial for proper translation and protein expression.
Formulation: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 1mM EDTA, 0.1M NaCl
Purity: > 90% by SDS - PAGE
Concentration: 0.5 mg/ml (determined by Bradford assay)
Sequences of animo acids: MGSSHHHHHH SSGLVPRGSH MPAAECATQR APGSVVELLG KSYPQDDHSN LTRKVLTRVG RNLHNQQHHPLWLIKERVKE HFYKQYVGRFGTPLFSVYDN LSPVVTTWQN FDSLLIPADH PSRKKGDNYY LNRTHMLRAH TSAHQWDLLH AGLDAFLVVG DVYRRDQIDS QHYPIFHQLE AVRLFSKHEL FAGIKDGESL QLFEQSSRSA HKQETHTMEA VKLVEFDLKQ TLTRLMAHLF GDELEIRWVD CYFPFTHPSF EMEINFHGEWLEVLGCGVME QQLVNSAGAQ DRIGWAFGLG LERLAMILYD IPDIRLFWCE DERFLKQFCV SNINQKVKFQ PLSKYPAVIN DISFWLPSEN YAENDFYDLV RTIGGDLVEK VDLIDKFVHP KTHKTSHCYR ITYRHMERTL SQREVRHIHQ ALQEAAVQLL GVEGRF
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
Gene Name: FARS2 phenylalanyl-tRNA synthetase 2, mitochondrial [ Homo sapiens ]
Synonyms: FARS2; phenylalanyl-tRNAsynthetase 2, mitochondrial; FARS1; PheRS; HSPC320; dJ520B18.2;phenylalanyl-tRNAsynthetase, mitochondrial; phenylalanine-tRNAsynthetase 1 (mitochondrial);dJ236A3.1;phenylalanine tRNA ligase 2, mitochondrial;phenylalanine translase;phenylalanine--tRNA ligase;phenylalanine-tRNAsynthetase 1 (mitochondrial);phenylalanine-tRNAsynthetase 2 (mitochondrial);dJ520B18.2 (FARS1 (phenylalanine-tRNAsynthetase)); EC6.1.1.20;HSPC320;dJ236A3.1;Phenylalanine--tRNA ligase;phenylalanine tRNA ligase 2, mitochondrial;phenylalanine translase
Gene ID: 10667
MIM: 611592
mRNA Refseq: NM_006567
Protein Refseq: NP_006558
UniProt ID: O95363
Chromosome Location: 6p25.1
Function: ATP binding;ligase activity;magnesium ion binding;nucleotide binding;phenylalanine-tRNA ligase activity;phenylalanine-tRNA ligase activity;tRNA binding

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