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Active Recombinant Human MAM Domain Containing Glycosylphosphatidylinositol Anchor 1, His-tagged

Cat.No. : MDGA1-5686H
Product Overview : Recombinant Human MDGA1protein was expressed in Murine myeloma cell line with a C-terminal 6-His tag. Gln19-Gly931.
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Cat. No. : MDGA1-5686H
Description : MDGA1 is a 140 kDamember of the Ig superfamily of proteins. Human MDGA1 is synthesized as a 955amino acid (aa) precursor that contains an 18 aa signal sequence, a 914 aamature chain, and a 23 aa propeptide. The mature chain consists of sixIg-like domains, a fibronectin type III domain (aa 640-739), a MAM domain (aa751-918), 11 potential sites for N-linked glycosylation, and a GPI anchor (aa932). Alternative splicing in human MDGA1 produces an isoform variant thatshows a 48 aa substitution for aa corresponding to positions 926-955.
Source : Mammalian cells
Form : Lyophilized from a0.2 μm filtered solution in PBS.
N-terminalSequence : Gln19 predicted
Molecular Weight : 101.9kDa
Activity : Measuredby its ability to enhance neurite outgrowth of E18 rat embryonic corticalneurons. Able to significantly enhance neurite outgrowth when immobilized at7.5-30 μg/Ml on a nitrocellulose-coated microplate.
Endotoxin Level : <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE : 120-140kDa, reducing conditions.
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : 500μg/mL in sterile PBS
Storage : Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
Gene Name : MDGA1 MAM domain containingglycosylphosphatidylinositol anchor 1 [ Homo sapiens ]
Official Symbol : MDGA1
Synonyms : MDGA1; MAM domaincontaining glycosylphosphatidylinositol anchor 1; GPIM; MAMDC3; FLJ45018;DKFZp686K0262; DKFZp686L0262; MAM domain-containingglycosylphosphatidylinositol anchor protein 1; GPI and MAM protein; MAMdomain-containing protein 3; glycosylphosphatidylinositol-MAM;glycosyl-phosphatidyl-inositol-MAM; OTTHUMP00000016331; OTTHUMP00000016332
Gene ID : 266727
mRNA Refseq : NM_153487
Protein Refseq : NP_705691
MIM : 609626
UniProt ID : Q8NFP4
Chromosome Location : 6p21
Function : molecular function

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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