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Active Recombinant Mouse Hepatocyte Growth Factor, His-tagged

Cat.No. : Hgf-728M
Product Overview : Recombinant Mouse Hgfprotein was expressed in Murine myeloma cell line with a C-terminal 10-Histag. Met1-Ser653. The proform was purified, activated and further purified.
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Cat. No. : Hgf-728M
Description : Hgf is a serineendopeptidase that cleaves at the peptide bond between Arg494 and Val495 ofsingle-chain human Hgf precursor, generating the active heterodimer. Hgf isproduced and secreted by the liver and normally circulates in the blood as aninactive zymogen. The zymogen has a weak affinity for heparin but acquires astrong affinity for heparin upon activation that is linked to bloodcoagulation.
Source : Mammalian cells
Form : Supplied as a 0.2 μmfiltered solution in Tris, NaCl and CaCl2.
N-terminalSequence : Val370 & Ile406
Molecular Weight : 32 kDa & 28 kDa
Activity : Measuredby its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 100 pmoles/min/μg, as measured underthe described conditions.
Endotoxin Level : <1.0 EUper 1 μg of the protein by the LAL method.
SDS-PAGE : 35 kDaand 32 kDa, reducing conditions.
Purity : >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage : Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 6 months fromdate of receipt, -70°C as supplied. 3 months, -70°C under sterile conditionsafter opening.
Gene Name : Hgf hepatocyte growth factor [ Musmusculus ]
Official Symbol : Hgf
Synonyms : Hgf; hepatocytegrowth factor; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik; SF; scatter factor;hepatopoeitin-A
Gene ID : 15234
mRNA Refseq : NM_010427
Protein Refseq : NP_034557
UniProt ID : Q08048
Chromosome Location : 5 A2-A3; 5 4.0 cM
Pathway : Cytokine-cytokinereceptor interaction; Focal Adhesion; Focal adhesion; Hemostasis; Malaria;Melanoma; Pathways in cancer; Platelet activation; Platelet degranulation;Renal cell carcinoma; Response to elevated platelet cytosolic Ca2+;Signaling of Hepatocyte Growth Factor Receptor
Function : catalytic activity;growth factor activity; protein heterodimerization activity; serine-typeendopeptidase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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