||Recombinant human RBM8A protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
||RBM8A is a member of the EJC(The exon junction complex) that is involved in mRNA export, cytoplasmic localization and nonsense mediated mRNA decay. This protein has the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. Although it shuttles to the cytoplasm, it is predominantly detected in the nucleus and is colocalized with oskar mRNA at the posterior pole of the cell.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol; 0.1M NaCl,1mM DTT
||20.9 kDa(182aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIHDKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRLEHHHH HH
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.