||Recombinant human S100A16 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||S100 calcium binding protein A16, also known S100A16, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Intracellularly, S100A16 is both nuclear and cytoplasmic and apparently exists as a homodimer, trimer and tetramer. It contains two EF-hand motifs with only one high-affinity Ca-binding site and apparently undergoes phosphorylation at Ser103.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol,0.2M NaCl
||13.9 kDa (123aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSDCYTELEK AVIVLVENFY KYVSKYSLVK NKISKSSFRE MLQKELNHML SDTGNRKAAD KLIQNLDANHDGRISFDEYW TLIGGITGPI AKLIHEQEQQ SSS
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.