||Recombinant human NPL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography(1-320aa).
||NPL, also known as N-acetylneuraminate lyase, is an enzyme that catalyzes the chemical reaction. (N-acetylneuraminate ->N-acetyl-D-mannosamine + pyruvate) This protein belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. It participates in amino sugars metabolism.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
||37.3 kDa (340aa) confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAFPKKKLQG LVAATITPMT ENGEINFSVI GQYVDYLVKE QGVKNIFVNG TTGEGLSLSV SERRQVAEEWVTKGKDKLDQ VIIHVGALSL KESQELAQHA AEIGADGIAV IAPFFLKPWT KDILINFLKE VAAAAPALPF YYYHIPALTG VKIRAEELLDGILDKIPTFQ GLKFSDTDLL DFGQCVDQNR QQQFAFLFGV DEQLLSALVM GATGAVGSTY NYLGKKTNQM LEAFEQKDFS LALNYQFCIQRFINFVVKLG FGVSQTKAIM TLVSGIPMGP PRLPLQKASR EFTDSAEAKL KSLDFLSFTD LKDGNLEAGS
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.