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Recombinant Human Glucose-6-phosphate Dehydrogenase, His-tagged

Cat. No.: G6PD-2397H
Product Overview: Recombinant humanG6PD protein, fused to His-tag at N-terminus, was expressed in Hi-5 cellusing baculovirus expression system and purified by using conventionalchromatography
Description: Glucose-6-phosphatedehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphatepathway, a metabolic pathway that supplies reducing energy to cells bymaintaining the level of NADPH. G6PD converts glucose-6-phosphate into6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH inturn maintains the level of glutathione in these cells that helps protect thered blood cells against oxidative damage. G6PD deficiency cause acutehemolytic anemia
Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol 0.1mM PMSF, 2mM EDTA, 2mMDTT, 200mM NaCl
Molecular Weight: 61.4kDa (535aa)
Purity: > 95% by SDS -PAGE
Concentration: 0.5mg/ml (determinedby Bradford assay)
Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGLLPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHLGSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRIDHYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCLVAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAVVLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPGMFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPIPYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
Biological activity: Specific activity is > 7 units/mlobtained by measuring the increase of NADPH in absorbance at 340 nm resultingfrom the reduction of NAD or NADP. One unit oxidizes 1.0 umoleD-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence ofbeta-NADP at pH 7.4 at 25C
Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.
Officialsymbol: G6PD
Gene Name: G6PD glucose-6-phosphatedehydrogenase [Homo sapiens]
Synonyms: glucose-6-phosphatedehydrogenase; G6PD1; EC 1.1.1.49; OTTHUMP00000196180; glucose-6-phosphate1-dehydrogenase; glucose-6-phosphate dehydrogenase, G6PD; OTTHUMP00000026034;OTTHUMP00000026039
Gene ID: 2539
mRNA Refseq: NM_001042351
Protein Refseq: NP_001035810
MIM: 305900
UniProt ID: P11413
Chromosome Location: Xq28
Pathway: Glutathionemetabolism; Glutathione metabolism; Glutathione metabolism; Metabolicpathways; Metabolism of carbohydrates; Pentose Phosphate Pathway
Function: NADPbinding; binding; glucose binding; glucose binding; glucose-6-phosphatedehydrogenase activity; oxidoreductase activity; protein homodimerizationactivity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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