||Recombinant humanTCEA1 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||TCEA1 (Transcriptionelongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds toPol II and functions to cleave the nascent transcript, thereby unlocking thecomplex and allowing transcription to continue. Localized to the nucleus,TCEA1 contains three independently-folding domains, all of which arenecessary for proper binding to Pol II. The arresting sites in DNA have theproperty of trapping a certain fraction of elongating RNA polymerases thatpass through, resulting in locked ternary complexes. Cleavage of the nascenttranscript by S-II allows the resumption of elongation from the new3"-terminus.
||Liquid. In 20 mMTris-HCl buffer, pH7.5, 20% glycerol, 1mM DTT, 50mM NaCl
||36.5kDa (325aa),confirmed by MALDI-TOF
||> 90% by SDS -PAGE
||1mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQSTDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTYVSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRISNLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKCKKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.