||Recombinanthuman GSTA4 protein, fused to His-tag at N-terminus, was expressed in E. coliand purified by using conventional chromatography techniques.
||GSTA4 belongs to the GST superfamily. Thisenzyme is involved in cellular defense against toxic, carcinogenic, andpharmacologically active electrophilic compounds. GSTA4 shows very highactivity with reactive carbonyl compounds such as alk-2-enals. GSTA4 ishighly effective in catalyzing the conjugate addition of reduced glutathioneto 4-hydroxynonenal, an important product of peroxidative degradation ofarachidonic acid and a commonly used biomarker for oxidative damage intissue. This enzyme is expressed at a high level inbrain, placenta, and skeletal muscle and much lower in lung and liver.
||Liquid. In 20 mMTris-HCl buffer, pH 8.0, 20% glycerol, 2mM DTT, 100mM NaCl.
||28.3 kDa (246aa)confirmed by MALDI-TOF
||> 95% by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQDGNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDDQQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTVKLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
|Pathway||Biological oxidations; Drugmetabolism-cytochrome P450; Glutathione conjugation; Glutathione metabolism;Metabolism; Metabolism of xenobiotics by cytochrome P450; Phase IIconjugation|