||Recombinant humanUBA5 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||UBA5(ubiquitin-like modifier activating enzyme 5) belongs to theubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin andubiquitin-like proteins are known to be covalently conjugated to a variety ofcellular substrates via a three-step enzymatic pathway. Theubiquitin-activating enzyme (E1) plays a key role in the first step ofubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5had been proved to activate an ubiquitin-like protein, ubiquitin-foldmodifier 1 (Ufm1), by forming a high-energy thioester bond. It localizesmainly in cytoplasm, while it mainly localizes to the nucleus in presence ofSUMO2.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl.
||47.4kDa(428aa) confirmed by MALDI-TOF.
||> 90%by SDS-PAGE
||0.5mg/ml (determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSEVVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRLFFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVEN FQHFMDRISN GGLEEGKPVD LVLSCVDNFEARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPTTMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQEVIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLEDLMAKMKNM
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.