||Recombinant mouse S100a5protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||S100calcium binding protein A5, also known as S100a5, is a member of the S100 family of proteins containing 2EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasmand nucleus of a wide range of cells, and involved in the regulation of anumber of cellular processes such as cell cycle progression anddifferentiation. S100a5 has a Ca2+ affinity20- to 100-fold higher than the other S100 proteins studied under identicalconditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly whichimpairs the binding of Ca2+. It is expressed in very restricted regions ofthe adult brain.
||Liquid. In 20 mMTris-HCl buffer, pH8.0, 40% glycerol, 3mM DTT, 200mM NaCl.
||13.4kDa(117aa), confirmed by MALDI-TOF.
||> 95%by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMETPLE KALTTMVTTF HKYSGREGSK LTLSRKELKE LIKTELSLAEKMKESSIDNL MKSLDKNSDQ EIDFKEYSVF LTTLCMAYND FFLEDNK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.