||Recombinant humanFABP9 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||FABP9(Fatty acid binding protein 9), also known as PERF15 or T-FABP, is a memberof fatty acid-binding proteins (FABPs) which are a family of small, highlyconserved, cytoplasmic proteins to bind long-chain fatty acids and otherhydrophobic ligands. It is found in midpachytene spermatocytes and roundspermatids, and constitutes part of the perinuclear theca. Functionally,FABP9 is likely to link intracellular membranes, and may signal abnormalsperm formation during spermatogenesis.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol.
||17.6 kDa(156aa), confirmed by MALDI-TOF.
||> 95%by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMVEPFL GTWKLVSSEN FEDYMKELGV NFAARNMAGL VKPTVTISVDGKMMTIRTES SFQDTKISF KLGEEFDETTA DNRKVKSTIT LENGSMIHVQKWLGKETTIK RKIVDEKMVV ECKMNNIVST RIYEKV
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.