||Recombinant humanGSTO2 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||GlutathioneS-transferase omega 2, also known as GSTO2, belongs to the GST superfamilyand may be involved in catalyzing the reaction of glutathione with a widevariety of organic compounds to form thioethers, a process that is essentialfor the metabolism and detoxification of a variety of xenobiotics andcarcinogens. GSTO2 is related to GSTO1 and is expressed in a variety oftissues throughout the body where it functions to catalyze the conversion ofRX and glutathione to HX and R-S-glutathione.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 40% glycerol.
||30.6 kDa(266aa), confirmed by MALDI-TOF.
||> 85%by SDS-PAGE
||0.25mg/ml (determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSMSGDATR TLGKGSQPPG PVPEGLIRIY SMRFCPYSHR TRLVLKAKDIRHEVVNINLR NKPEWYYTKHPFGHIPVLET SQCQLIYESV IACEYLDDAY PGRKLFPYDP YERARQKMLLELFCKVPHLT KECLVALRCG RECTNLKAAL RQEFSNLEEI LEYQNTTFFG GTCISMIDYL LWPWFERLDVYGILDCVSHT PALRLWISAM KWDPTVCALL MDKSIFQGFL NLYFQNNPNA FDFGLC
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.