||Recombinant Human ALDH8A1protein was expressed as gst-tagged fusion protein by E. coli and purified byGSH-sepharose. The purified protein was resolved in 1M PBS (58mM Na2HPO4, 17mMNaH2PO4, 68mM NaCl, pH8.) added with 100mM GSH and 1% TritonX-100,15%glycerol.
||ALDH8A1 belongs tothe aldehyde dehydrogenase family of proteins. It plays a role in a pathway of9-cis-retinoic acid biosynthesis in vivo. ALDH8A1 converts 9-cis-retinal intothe retinoid X receptor ligand 9-cis-retinoic acid, and has approximately40-fold higher activity with 9-cis-retinal than with all-trans-retinal.Therefore, it is the first known aldehyde dehydrogenase to show a preferencefor 9-cis-retinal relative to all-trans-retinal. Two transcript variantsencoding distinct isoforms have been identified for this gene.
||Theprotein is stored in PBS buffer at -20°C.Repeated freeze-thaw cycles should be avoided.