Recombinant Human PPM1G, His-tagged

• Cat.No.: PPM1G-3564H
• Product Overview: Recombinant humanPPM1G protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.

Specification

• Cat. No.: PPM1G-3564H
• Description: PPM1G,as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thrprotein phosphatases that is negative regulators of cell stress responsepathways. This enzyme localizes to the cytoplasm and is widely expressed,with most abundant expression detected in testis, skeletal muscle and heart.It is necessary for the dephosphorylation of pre-mRNA splicing factors, whichis an important process for the formation of the functional spliceosome.
• Form: Liquid. 20mM Tris-HClbuffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF.
• Molecular Weight: 61.4 kDa(566aa)
• Purity: > 85%by SDS - PAGE
• Concentration: 1.0mg/ml (determined by BCA assay)
• Biological activity: Specificactivity: >3,000 units/mg. Enzymatic activity was confirmed by measuringthe amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP)per minute at 37C, pH7.5 using 10mM of substrate.
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYDGHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKL TTEEV IKELAQIAGR PTEDEDEKEKVADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETPSQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEEEEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQLIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKNLPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELLDQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PPM1G protein phosphatase,Mg2+/Mn2+ dependent, 1G [ Homo sapiens ]
• Official Symbol: PPM1G
• Synonyms: PPM1G; protein phosphatase, Mg2+/Mn2+dependent, 1G; PP2CG; PPP2CG; PP2CGAMMA; protein phosphatase 1G; PP2C-gamma;PP2C, gamma; MGC1675; protein phosphatase 1C; protein phosphatase 2C gammaisoform; protein phosphatase 2C isoform gamma; protein phosphatasemagnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gammaisoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gammaisoform; EC 3.1.3.16; OTTHUMP00000123426
• Gene ID: 5496
• mRNA Refseq: NM_177983
• Protein Refseq: NP_817092
• MIM: 605119
• UniProt ID: O15355
• Chromosome Location: 2p23.3
• Pathway: mRNA processing
• Function: catalytic activity; hydrolase activity;metal ion binding; protein serine/threonine phosphatase activity