||Recombinant human OTUB2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-234aa).
||OTUB2 (Ubiquitin thioesterase OTUB2) belongs to the peptidase C65 family. OTUB2 functions as a hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol, 50mM NaCl
||29.4kDa (254aa) confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSETSFNLIS EKCDILSILR DHPENRIYRR KIEELSKRFTAIRKTKGDGN CFYRALGYSY LESLLGKSRE IFKFKERVLQ TPNDLLAAGF EEHKFRNFFN AFYSVVELVE KDGSVSSLLK VFNDQSASDH IVQFLRLLTS AFIRNRADFF RHFIDEEMDI KDFCTHEVEP MATECDHIQI TALSQALSIA LQVEYVDEMD TALNHHVFPE AATPSVYLLYKTSHYNILYA ADKH
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.