||Recombinant humanUBL3 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||UBL3,also known as PNSC1, is a 117 amino acid membrane protein belonging to theubiquitin-like family. This protein contains two potential N-glycosylationsites, a potential protein kinase C phosphorylation site and a potential C-terminalprenylation site. Ubiquitin-like proteins are not directly involved inprotein degradation, but appear to have many mechanistic similarities withthe ubiquitin pathway.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl.
||15.0 kDa(134 aa), confirmed by MALDI-TOF.
||> 90%by SDS - PAGE
||1 mg/ml (determinedby BCA)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MSSNVPADMI NLRLILVSGK TKEFLFSPND SASDIAKHVY DNWPMDWEEEQVSSPNILRL IYQGRFLHGN VTLGALKLPF GKTTVMHLVA RETLPEPNSQ GQRNREKTGE SNCC
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.