||Recombinant humanUBL5 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||UBL5,also known ubiquitin-like protein 5, is thought to be reversible modulatorsof protein function rather than protein degraders like ubiquitin. Thisprotein effects a nuclear step required for mounting a response to the threatof mitochondrial protein misfolding.
||Liquid. 20mM Tris-HClbuffer (pH8.0) containing 20% glycerol 0.1M NaCl, 2 mM DTT.
||10.7 kDa(93aa), confirmed by MALDI-TOF.
||> 90%by SDS - PAGE
||0.5 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MIEVVCNDRL GKKVRVKCNT DDTIGDLKKL IAAQTGTRWNKIVLKKWYTI FKDHVSLGDY EIHDGMNLEL YYQ
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.