||Recombinant human PTGES2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-186aa).
||PTGES2 is a membrane-associatedprostaglandin E synthase, which catalyzes the conversion ofprostaglandin H2 to prostaglandin E2. This protein also has beenshown to activate the transcription regulated by agamma-interferon-activated transcription element.
||23.5 kDa (206aa), confirmed by MALDI-TOF
||Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 20% glycerol, 2mM DTT, 0.2M NaCl.
||> 95 % by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MKAVNEQGKE VTEFGNKYWL MLNEKEAQQV YGGKEARTEE MKWRQWADDW LVHLISPNVY RTPTEALASF DYIVREGKFG AVEGAVAKYM GAAAMYLISK RLKSRHRLQD NVREDLYEAA DKWVAAVGKD RPFMGGQKPN LADLAVYGVL RVMEGLDAFD DLMQHTHIQP WYLRVERAIT EASPAH
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.