||Recombinant human UROS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-265aa).
||UROS, also known as Uroporphyrinogen III synthase, is an enzyme involved in the fourth step of porphyrin metabolism, involved in the conversion of hydroxymethylbilane into uroporphyrinogen III. Defects in this protein can cause molecular lesions that lead to the autosomal recessive Gunther disease, also known as congenital erythropoietic porphyria (CEP).
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 0.1M NaCl
||30.7 kDa(285aa) confirmed by MALDI-TOF
||>95% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MKVLLLKDAK EDDCGQDPYI RELGLYGLEA TLIPVLSFEF LSLPSFSEKL SHPEDYGGLI FTSPRAVEAAELCLEQNNKT EVWERSLKEK WNAKSVYVVG NATASLVSKI GLDTEGETCG NAEKLAEYIC SRESSALPLL FPCGNLKREI LPKALKDKGIAMESITVYQT VAHPGIQGNL NSYYSQQGVP ASITFFSPSG LTYSLKHIQE LSGDNIDQIK FAAIGPTTAR ALAAQGLPVS CTAESPTPQALATGIRKALQ PHGCC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.