Recombinant Human IDH1 protein, His-tagged

• Cat.No.: IDH1-2527H
• Product Overview: Recombinant Human IDH1 (Met1-Leu414) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Isocitrate dehydrogenase 1 (IDH1) is also known as Cytosolic NADP-isocitrate dehydrogenase (NADP(+)-specific ICDH), Oxalosuccinate decarboxylase and PICD, which is one of the Isocitrate dehydrogenases that can catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD+ as the electron acceptor and the other NADP+. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
• Source: E.coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 0.05% Brij-35, 1 mM DTT, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 47.5 kDa. The protein migrates as 47.5 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Met1-Leu414
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IDH1
• Official Symbol: IDH1
• Synonyms: IDH1; isocitrate dehydrogenase 1 (NADP+), soluble; isocitrate dehydrogenase [NADP] cytoplasmic; NADP(+)-specific ICDH; oxalosuccinate decarboxylase; NADP-dependent isocitrate dehydrogenase, cytosolic; NADP-dependent isocitrate dehydrogenase, peroxisomal; IDH; IDP; IDCD; IDPC; PICD
• Gene ID: 3417
• mRNA Refseq: NM_005896
• Protein Refseq: NP_005887
• MIM: 147700
• UniProt ID: O75874